A method of PCB metabolite determination in anaerobic freshwater sediments using a upflow anaerobic biofiltration process (ANBIOF) Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/dv13zw570

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  • The biodegradation of five polychlorinated biphenyls (PCBs) by Hudson River and Silver Lake sediments was studied using gas chromatography with electron capture detection (GC/ECD) and gas chromatography with mass spectrometry (GC/MS) analyses. Methanogenic sediments from the Hudson River and Silver Lake were used to inoculate four anaerobic filter reactors. The upflow anaerobic filters were fed a synthetic wastewater containing the five PCB congeners, semicontinuously, at a 10 day hydraulic retention time. The PCBs were fed at increasing concentrations to stimulate bacterial degradation and metabolite formation. The production of an unknown metabolite was detected by observing the formation of a unique peak on the gas chromatogram. The peak clearly indicated a dependence on reactor sediment parent PCB concentrations by exhibiting a nine fold increase in peak area during a ten fold increase in parent compound feed concentrations. GC/MS analyses confirmed the unknown peak as a tetrachlorobiphenyl (tetra-CB), and cochromatography identified the tetra-CB as 2,3,5,6-tetrachlorobiphenyl (2356-CB). Of the five congeners studied, 2,3,4,5,6-pentachlorobiphenyl (23456-CB) was clearly biodegraded. The parent compound, 23456-CB, was reductively dechlorinated at the para position to produce a 2356-CB. Reactors inoculated with Hudson River sediments exhibited dechlorination at an apparent ten fold lower sediment parent PCB concentration and at shorter lag times than observed for Silver Lake sediments. The results indicate anaerobic biodegradative competence exists for 23456 CB in Hudson River and Silver Lake sediments.
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