Suppression of yolk polypeptide gene expression in Drosophila melanogaster by sodium butyrate treatment Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/dz010s92z

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Current evidence suggests that temporal regulation of gene expression in eucaryotic organisms relies primarily upon localized structural modifications of chromatin. One possible means of changing chromatin structure is through varying the level of acetylation of the histone proteins. Sodium butyrate has been found to inhibit the activity of histone deacetylase enzymes causing hyperacetylation of the histones. Butyrate treatment was used as a means of examining the role of chromatin structure in the hormonal regulation of the yolk polypeptide (YP) genes in Drosophila melanogaster. Treatment of newly eclosed adult females with doses of butyrate up to 50 mM did not significantly affect the quantity of YPs produced 24 hours after the treatment. Similarly, doses of 5 and 10 mM butyrate had no effect on YP production between 12 and 48 hours after treatment with butyrate. Mature adult female Drosophila, at maximal YP production, maintained normal production of YPs after treatment with butyrate at doses up to 50 mM. Doses greater than 50 mM sodium butyrate resulted in toxic effects, evidenced by increased morbidity, which were related to the molar concentration of sodium rather than to the molar concentration of butyrate. Adult males were more sensitive to the butyrate treatments than females since morbidity was about 30% in males treated with 50 mM butyrate compared to 10% for females at the same dose. The effects of the butyrate on females were transient since mated, butyrate treated females laid normal numbers of eggs which developed into normal offspring. When butyrate was administered concomitantly with either 20-hydroxyecdysone or a juvenile hormone analogue to female abdomens isolated from the anterior endocrine organs at eclosion, the hormonal stimulation of YP synthesis was not affected. This study, which found no detectable effect of butyrate on YP gene expression in Drosophila melanogaster, was more limited in scope than the majority of studies on preparations of other organisms which demonstrated marked alterations in the expression of specific genes following butyrate treatment. Therefore, a full characterization of the effects of butyrate on YP gene expression would require additional studies regarding the metabolism of butyrate by Drosophila, the effect of butyrate on the acetylation of core histones of Drosophila, and the effect of butyrate on transcription of the YP genes into messenger RNA.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 8-bit Grayscale) using ScandAll PRO 1.8.1 on a Fi-6770A in PDF format. CVista PdfCompressor 5.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2013-07-18T17:05:50Z (GMT). No. of bitstreams: 1 FairfaxCarolynS1985.pdf: 1032144 bytes, checksum: 35ca81395110d431e80daeff65b8e67b (MD5) Previous issue date: 1984-08-27
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-07-16T22:07:20Z (GMT) No. of bitstreams: 1 FairfaxCarolynS1985.pdf: 1032144 bytes, checksum: 35ca81395110d431e80daeff65b8e67b (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-07-18T17:05:50Z (GMT) No. of bitstreams: 1 FairfaxCarolynS1985.pdf: 1032144 bytes, checksum: 35ca81395110d431e80daeff65b8e67b (MD5)
  • description.provenance : Submitted by Sergio Trujillo (jstscanner@gmail.com) on 2013-07-16T18:06:57Z No. of bitstreams: 1 FairfaxCarolynS1985.pdf: 1032144 bytes, checksum: 35ca81395110d431e80daeff65b8e67b (MD5)

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items