Genomic characterization of a novel leporid Herpes simplex virus Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/f1881p143

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • The viral family Herpesviridae consists of large double stranded DNA viruses including eight species that infect humans with varying pathology from benign rashes to cancerous cell transformation. From three subfamilies, alpha-, beta- and gammaherpes, the alphaherpes contains the genera iltovirus, mardivirus, varicellovirus and simplex, two of which, the human simplex viruses I and 2 (HSV) induce life-long infections that have appeared to have coevolved with their hosts from the origins of our species. Unique features of the simplex genus are latency, tropism in dorsal root ganglia neurons, extraordinary high GC content ranging from 65 to 77%, and nucleosome formation of their genomes within the host's nucleus without integration. Reviewing the basic molecular and genetic characteristics of herpes simplex will introduced in Chapter 1, followed by the introduction of a newly sequenced, de novo assembled and predicatively annotated herpes simplex virus, Leporid Herpes Virus-4 (LHV4). Isolated from a virulent outbreak in domesticated rabbits, LHV4 has the smallest reported simplex virus to date at roughly 125,600 base pairs and presents similar pathology seen in rabbit models infected with HSV. Comparative genomics revealed a high degree of sequence similarity and genome synteny between LHV4 and other simplex viruses. Four genes were not computationally predicted in our annotation and may be absent in the LHV4 genome. The absent proteins correspond to: UL56, ICP34.5, US5 and US12 and have postulated roles in membrane trafficking, neurovirulence, apoptotic control and MHC I presentation respectively. The solved genome structure leads to how this compacted genome functions with the noted absences to produce a similar pathology in rabbits to that of HSV and whether other biological correlates will continue to be found in in vitro and in vivo infection. The inverted repeat regions (IR), duplicated and inverted to simplex virus' two larger blocks of protein-coding regions are described in Chapter 3. The similarities and differences in critical genes from the IR that balance latency and replicative viral cycles are compared. A two-fold reduction in IR content indicates the ability for a simplex virus to maintain infectivity despite this large truncation. The appendix describes the eukaryotic phylogeny of two initiating proteins of the mismatch repair (MMR) pathway. MMR proteins are present in the replicative foci of productive herpes virus infection and this analysis may indicate adaptive pressures involved in both genomic fidelity and host tropism. The emerging era of state-of-the-art genome sequencing and computational power advances this newly characterized herpes virus, along with its model host organism, as excellent candidates for systems interaction, and experimental biology.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Non-Academic Affiliation
Keyword
Subject
Rights Statement
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Submitted by Bobby Babra (babrab@onid.orst.edu) on 2012-01-25T22:07:31Z No. of bitstreams: 1 BabraBobbyA2012.pdf: 11144861 bytes, checksum: 31cea8eddef69b447a07762468c5104f (MD5)
  • description.provenance : Approved for entry into archive by Julie Kurtz(julie.kurtz@oregonstate.edu) on 2012-01-26T18:02:15Z (GMT) No. of bitstreams: 1 BabraBobbyA2012.pdf: 11144861 bytes, checksum: 31cea8eddef69b447a07762468c5104f (MD5)
  • description.provenance : Approved for entry into archive by Laura Wilson(laura.wilson@oregonstate.edu) on 2012-01-27T16:55:38Z (GMT) No. of bitstreams: 1 BabraBobbyA2012.pdf: 11144861 bytes, checksum: 31cea8eddef69b447a07762468c5104f (MD5)
  • description.provenance : Made available in DSpace on 2012-01-27T16:55:38Z (GMT). No. of bitstreams: 1 BabraBobbyA2012.pdf: 11144861 bytes, checksum: 31cea8eddef69b447a07762468c5104f (MD5) Previous issue date: 2012-01-05

Relationships

In Administrative Set:
Last modified: 08/03/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items