The response of nitrifying bacteria to treatments of N-Serve and Roundup in continuous-flow soil columns Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/f7623g23j

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • This study examined the effects of Roundup [N-(phosphonomethyl) glycine] and N-Serve [2-chloro-6- (trichloromethyl) pyridine] on nitrifying organisms in static batch, perfusion soil columns, and a new continuous-flow soil column system. The continuous-flow method is new to nitrification studies and was shown to produce greater nitrifier activity than either static batch of perfusion techniques. Both N-Serve and Roundup were shown to significantly inhibit nitrification in treated soils over untreated controls. N-Serve completely inhibited nitrification at concentrations greater than 42 ug nitrapyrin g⁻ ¹ dry soil. Roundup significantly reduced nitrification at 6.8 and 68 mg glyphosate g⁻ ¹ dry soil. Continuous-flow columns were examined to determine if microbial populations were altered by chemical treatment. Concentrations of 4.2 mg nitrapyrin and 68 mg glyphosate g⁻ ¹ dry soil significantly increased heterotrophic populations. Numbers of heterotrophs were not significantly different from controls in soils at lower concentration. Numbers of nitrifying bacteria did not appear to change following treatment although nitrification was inhibited. Fluorescent antibody analysis of nitrifiers revealed that Nitrosolobus was more numerous than Nitrosospira and Nitrosomonas. Nitrosolobus increased in number whereas the other two genera remained unchanged. In this study, the continuous-flow system proved to be both reliable and useful in the culture of nitrifying bacteria. This method is an alternative to traditional techniques for evaluation of the effects of chemicals on microbial biogeochemical cycles.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2013-07-08T22:59:24Z (GMT). No. of bitstreams: 1 RhodesAlbertN1989.pdf: 388025 bytes, checksum: 42c71f527141b2975def6c46d882384b (MD5) Previous issue date: 1988-07-15
  • description.provenance : Submitted by Katy Davis (kdscannerosu@gmail.com) on 2013-05-17T21:35:56Z No. of bitstreams: 1 RhodesAlbertN1989.pdf: 388025 bytes, checksum: 42c71f527141b2975def6c46d882384b (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-05-29T15:47:56Z (GMT) No. of bitstreams: 1 RhodesAlbertN1989.pdf: 388025 bytes, checksum: 42c71f527141b2975def6c46d882384b (MD5)
  • description.provenance : Approved for entry into archive by Deborah Campbell(deborah.campbell@oregonstate.edu) on 2013-07-08T22:59:24Z (GMT) No. of bitstreams: 1 RhodesAlbertN1989.pdf: 388025 bytes, checksum: 42c71f527141b2975def6c46d882384b (MD5)

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items