The effects of univalent cations on the inductive formation of nitrate reductase Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/fj236455m

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • An investigation was undertaken to determine whether potassium is indeed necessary for the in vivo synthesis of a specific protein. The effects of univalent cations on the inductive formation of nitrate reductase and on the level of activity of representative non-adaptive enzyme are presented. Cultures of Neurospora crassa were grown in media without added univalent cations and the mycelia then transferred to induction media containing different univalent cations. The inductive formation of nitrate reductase was followed over a four-hour period. During the same period of incubation assays for the activity of the constitutive enzyme pyruvic kinase were conducted. Results of nitrate reductase assays during the induction period indicate that the formation of this enzyme is dependent upon an adequate concentration of potassium in the medium. Sodium, lithium, and ammonium could not substitute for potassium in the inductive formation of this enzyme. Rubidium was the only univalent cation besides potassium that partially functioned in the synthesis of nitrate reductase. Studies of pyruvic kinase activity during the in duction period indicate that its activity remained nearly constant. It appears that conditions were optimum for the rapid synthesis of nitrate reductase, but the rate of protein synthesis in general was not greatly changed during the induction period of nitrate reductase.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 5.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Submitted by Georgeann Booth (gbscannerosu@gmail.com) on 2014-04-29T00:14:33Z No. of bitstreams: 1 NitsosRonald1966.pdf: 2134367 bytes, checksum: 2724cc43eb2d443408c06bac431c541b (MD5)
  • description.provenance : Rejected by Patricia Black(patricia.black@oregonstate.edu), reason: Replace on 2014-04-30T16:31:35Z (GMT)
  • description.provenance : Made available in DSpace on 2014-05-01T15:22:23Z (GMT). No. of bitstreams: 1 NitsosRonald1966.pdf: 2110436 bytes, checksum: f8d83af0f6562706fee2ac913f1dfa97 (MD5) Previous issue date: 1966-05-12
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2014-04-29T14:59:04Z (GMT) No. of bitstreams: 1 NitsosRonald1966.pdf: 2134367 bytes, checksum: 2724cc43eb2d443408c06bac431c541b (MD5)
  • description.provenance : Approved for entry into archive by Katy Davis(kdscannerosu@gmail.com) on 2014-04-30T16:49:42Z (GMT) No. of bitstreams: 1 NitsosRonald1966.pdf: 2110436 bytes, checksum: f8d83af0f6562706fee2ac913f1dfa97 (MD5)
  • description.provenance : Submitted by Georgeann Booth (gbscannerosu@gmail.com) on 2014-04-30T16:40:49Z No. of bitstreams: 1 NitsosRonald1966.pdf: 2110436 bytes, checksum: f8d83af0f6562706fee2ac913f1dfa97 (MD5)
  • description.provenance : Approved for entry into archive by Katy Davis(kdscannerosu@gmail.com) on 2014-05-01T15:22:23Z (GMT) No. of bitstreams: 1 NitsosRonald1966.pdf: 2110436 bytes, checksum: f8d83af0f6562706fee2ac913f1dfa97 (MD5)

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items