Chemical and genetic evaluation of high lysine and protein in selected barley crosses Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/fj2365695

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  • Four agronomically and genetically diverse spring barleys were used in a crossing program to study the inheritance of lysine in barley and to determine its possible association with certain agronomic and morphological characters. The cultivar Hiproly was used as the source of high protein and lysine. Characters measured included plant height, head type, awn type, lemma color, number of heads per plant, number of seeds per head, seed weight, and plant yield. Soluble protein content was determined by the Lowry colorimetric method. Lysine was analyzed by two methods: (1) enzyme hydrolysis and colorimetric determination with 2-chloro, 3, 5- dinitropyridine; (2) Udy dye-binding colorimetric method. The F₁, F₂, and F₃ progenies were classified for the Hiproly endosperm character by histochemical techniques using Udy Orange G dye as a staining agent. Fractionation of the soluble proteins into the Mendal- Osborn fraction was used to determine the effects of the Hiproly gene on kernel proteins. High lysine content in Hiproly was found to be inherited by one recessive gene. In addition several minor factors were indicated in all crosses. The recessive gene was associated with the recessive Hiproly endosperm gene with a recombination value of 22.45 percent. It is suggested that the gene designations for the two recessive genes be (lys) for the high lysine gene and (stb) for the starch binding endosperm gene. High lysine was negatively associated with protein content; however, the association was not strong enough to limit selection for high lysine, high protein lines. No associations were found between lysine and any of the morphological or agronomic characters studied. It was concluded that it is possible to breed high yielding, high protein and lysine barleys using Hiproly as a source of high protein and lysine. Other parental lines can be selected to complement the Hiproly gene to obtain even better nutritional balance.
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