Graduate Thesis Or Dissertation

 

The nature of the pentose phosphate pathway in strains of Saccharomyces cerevisiae Public Deposited

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  • The catabolism of glucose by proliferating and resting cells of two strains of Saccharomyces cerevisiae has been examined, with respect to the nature and participation of concurrent glucose pathways by means of the radiorespirometric method. The nicotinamide nucleotide content, and nicotinamide nucleotide oxidase activities in these cells were also examined. Data from the radiorespirometric experiments indicated that both strains of S. cerevisiae catabolized glucose by way of the EMPTCA and the PP pathway although relative participation of these two pathways were different. The pentose phosphate pathway is playing a much more important role in strain 2 of the bakers' yeast. Examination of the radiorespirometric pattern as well as the data on the incorporation of substrate carbon atoms into cellular constituents revealed that when bakers' yeast of strain 2 is permitted to utilize glucose under resting conditions, the data observed on the yields of respiratory C¹⁴O₂ cannot be used for the purpose of pathway estimation. This is true since under resting conditions, the slow rate of glucose utilization permits considerable interaction of glucose intermediates, thereby resulting in randomization of the two labeled carbon atoms. The contents of nicotinamide nucleotides as well as the activities of nicotinamide nucleotide oxidases in these cells were determined and found that they cannot be correlated with relative participation of glucose pathways.
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