Graduate Thesis Or Dissertation
 

Evaluation of a photon-counting technique for the reaction rate determination of glucose

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  • A photon-counting technique which provides direct digital read-out of data has been developed and evaluated for the reaction rate determination of glucose. The enzyme catalyzed oxidation of glucose to gluconic acid and hydrogen peroxide with the subsequent reaction of hydrogen peroxide with excess iodide to produce tri-iodide is followed by the change in the absorbance of the solution. Equations are derived which relate the photometer response to the rate of change of the tri-iodide concentration in the solution. Optimum results for high or low glucose concentration ranges were obtained with a low or high enzyme concentration respectively. Methods for obtaining linear calibration curves with or without using a blank solution are discussed. Linear calibration curves were obtained for glucose concentrations from 10-110 ppm with a reproducibility of about 2 ppm.
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