Evaluation of a photon-counting technique for the reaction rate determination of glucose Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/g445ch588

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  • A photon-counting technique which provides direct digital read-out of data has been developed and evaluated for the reaction rate determination of glucose. The enzyme catalyzed oxidation of glucose to gluconic acid and hydrogen peroxide with the subsequent reaction of hydrogen peroxide with excess iodide to produce tri-iodide is followed by the change in the absorbance of the solution. Equations are derived which relate the photometer response to the rate of change of the tri-iodide concentration in the solution. Optimum results for high or low glucose concentration ranges were obtained with a low or high enzyme concentration respectively. Methods for obtaining linear calibration curves with or without using a blank solution are discussed. Linear calibration curves were obtained for glucose concentrations from 10-110 ppm with a reproducibility of about 2 ppm.
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  • File scanned at 300 ppi using Capture Perfect 3.0 on a Canon DR-9050C in PDF format. CVista PdfCompressor 5.0 was used for pdf compression and textual OCR.
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