OpMNPV p87, a baculovirus capsid protein : genetic location, nucleotide sequence, transcriptional mapping and immunochemical characterization Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/g445ch812

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  • A capsid protein termed p87 of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (OpMNPV) was located to the HindIII-G fragment of the viral genome by Southern hybridization. A 3.6 kb segment of this region was sequenced and an 1872-nt open reading frame encoding the p 87 protein was identified and transcriptionally mapped by Northern blot and 3' S1 analysis. The p87 is a baculovirus late gene and transcription is initiated from the late gene consensus GTAAG sequence. A p87 gene fusion was constructed by fusing 1116 nucleotides of the p87 gene (encoding the C-terminal for 367 amino acids with a predicted molecular weight of 42 kDa) to the bacterial trpE gene. Polyclonal antibodies were produced and the p87 protein was examined by Western blot analysis and high levels of expression was found to occur at 36 hours post infection. Further analysis indicated, p87 is a component of purified OpMNPV PDV, BV virions and isolated capsids. The trpE-p87 antibody did not cross react with any AcMNPV specific proteins in AcMNPV infected Spodoptera frugiperda cells.
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