Ti plasmid coded protein in Agrobacterium tumefaciens Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/g732dc503

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  • Agrobacterium tumefaciens is a plant pathogenic bacteria which incites crown gall tumors. Although virulence has been associated with a 97-156 Mdalton "tumor-inducing" (Ti) plasmid, few potential plasmid gene products have been identified. The objective of this study was to identify plasmid-coded gene products through a comparison of protein fingerprints of virulent and avirulent isogenic strains. This required development of techniques for cell fractionation which could provide comparable protein samples from the various strains of bacteria and a reproducible high resolution method of protein characterization. The analytical system developed consisted of isoelectric focusing in the first dimension followed by SDS-polyacrylamide gel electrophoresis in the second dimension and was capable of reproducibly resolving nearly 500 proteins using this two-dimensional system. A. tumefaciens strain C58, which contains a nopaline Ti plasmid, produced five proteins detected neither in plasmid-free isogenic strains nor in A277. Strain A277, which contains the octopine plasmid, pTiB6 -806, produced three proteins detected neither in plasmid-free isogenic strains nor in C58. Strain A136, a plasmid-free derivative of C58, was deficient in two proteins found in the other three strains examined. The implications behind the presence of these proteins and their possible biological roles are discussed.
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