General studies of temperature effects on an obligate psychrophile Vibrio marinus Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/gf06g556h

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  • Vibrio marinus, strain MP-1, was shown to grow from pH 6.7 to 8.5, with an optimum at 7.3. Growth was inhibited below salinity 10%₀ and above 60%₀ . The upper temperature limit of growth was 20 C, and growth was very rapid with aeration at 15 C. Heating of the culture in Rila sea water at temperatures above 20 C caused severe viability loss which was inhibited by nutrient presence at 25 C. This loss was partially attributed to a thermally-induced membrane lesion. The melting point (Tm) of DNA was 89.0 C; the percent extractable lipid content of the culture did not change with growth temperature. A thermal lesion was shown to be a membrane rupture by analysis of the protein, RNA, DNA and amino acids which were released during heat shocking. Amino acid disproportionation occurred with increase in the time and temperature of heating, probably due to a change in the hydrophobic-hydrophilic environment induced by heating. The RNA released was both polymeric and degraded. Heating caused the release of protein which was enzymatically active. A method for obtaining partially purified malic dehydrogenase from this culture was described.
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