Comparisons of thymic cells in the developing and involuting rat thymus in vitro Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/gf06g644x

Descriptions

Attribute NameValues
Alternative Title
  • Comparison of thymic cells in the developing and involuting rat thymus in vitro
Creator
Abstract or Summary
  • In the rat thymus, four stages of development have been distinguished: (1) a newborn stage (Stage I), (2) an accelerated growth stage (Stage II), (3) an involuting stage (Stage III), and (4) an involuted stage (Stage IV) . There were three major types of thymic lymphoid cells: small (STL), medium (MTL), and large (LTL) thymic lymphoid cells. By Ficoll- Hypaque gradient centrifugation, MTL- and LTL-rich populations were isolated for comparative analysis. Growth kinetics studies revealed that the MTL were the most active in proliferation and the most responsive to Concanavalin A (Con A) activation. The LTL had lower Con A responsiveness, and the STL were the least active in both categories. Stage II cell populations were found to be the most proliferative and Con A reactive. Stage I and III cell populations were second in both categories of cellular response, while Stage IV cells were the least active in both proliferation and Con A activation. Stage III and IV cells also appeared to be more tolerant of high serum concentration than Stages I and II cells. Increases in cell concentration had synergistic effects on cellular proliferation and responsiveness to Con A activation. The suggested mechanism involves cell-to-cell mediation requiring intimate cell contact and possibly the formation of gap-junctions. The newborn MTL can tolerate a limited range of low temperature, and this tolerance is lost rapidly as a rat matures. On the other hand, an older rat has a higher proportion of long-lived MTL in its thymus. The proportion of reticuloepithelial cells (REC) decreased with age in the rat thymus, while the proportion of free macrophages remained unchanged. The number of lipoid cells increased with age, and was the result of REC and macrophage differentiation. Nonlymphoid cell and lymphoid cell rosettes in thymus explants were interpreted to be interacting macrophages and thymic lymphoid cells leading to the differentiation of the latter. Macrophages also served to remove debris and to restructure the involuting thymus.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 8-bit Grayscale) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Submitted by Katy Davis (kdscannerosu@gmail.com) on 2013-09-12T22:28:31Z No. of bitstreams: 1 TsuiDanielCN1979.pdf: 2147392 bytes, checksum: 32985cfe3fb2a339731544deee82bb23 (MD5)
  • description.provenance : Made available in DSpace on 2013-09-24T18:31:39Z (GMT). No. of bitstreams: 1 TsuiDanielCN1979.pdf: 2147392 bytes, checksum: 32985cfe3fb2a339731544deee82bb23 (MD5) Previous issue date: 1978-12-14
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-09-24T18:31:39Z (GMT) No. of bitstreams: 1 TsuiDanielCN1979.pdf: 2147392 bytes, checksum: 32985cfe3fb2a339731544deee82bb23 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-09-13T15:38:49Z (GMT) No. of bitstreams: 1 TsuiDanielCN1979.pdf: 2147392 bytes, checksum: 32985cfe3fb2a339731544deee82bb23 (MD5)

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items