Graduate Thesis Or Dissertation
 

Monolithic sorbents for microscale separations

Pubblico Deposited

Contenuto scaricabile

Scarica il pdf
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/gh93h2057

Descriptions

Attribute NameValues
Creator
Abstract
  • Over the last decade, the miniaturization of analytical systems has become an increasingly important and interesting research area. Miniaturized systems offer many advantages, including reduced reagent and sample consumption, shorter analysis times, portability and disposability. This dissertation describes novel approaches in this direction, focusing on two areas: the miniaturization of existing column chromatographic systems and the development of microfluidic systems in which the separation is performed in a channel on a microchip. A new type of methacrylate-based monolithic capillary columns for liquid chromatography and capillary electrochromatography were prepared within the confines of fused-silica tubing using Starburst dendrimers to affect porosity. The polyamidoamine (PAMAM) dendrimers were incorporated into a solution of functionalized monomer, cross-linker, solvents, and polymerization initiator. Thermal polymerization, followed by the removal of solvent and dendrimers, produced a continuous rod of polymer with uniform porosity. Different column porosities were obtained by varying the amount of the dendrimer template. The chromatographic performance of these monolithic columns was evaluated using a peptides mixture obtained by tryptic digestion of chicken egg lysozyme. A distinct advantage of polymer monolithic stationary phases over conventional packed chromatographic beds is the ability to prepare them easily and rapidly via free radical polymerization within the channels of a microfluidic device. In this work, continuous polymeric beds were prepared within a channel of three different microchip substrates: glass, poly(dimethylsiloxane) and polycarbonate. The methacrylate-based monolith was cast in-situ via UV-initiated polymerization. The functionalization of the inner wall of the channel with methacryloyl groups enabled the covalent binding of the monolith to the wall. The morphology of the wall-anchored monolith was studied by SEM of chip sections, and by SEM of an extruded segment of non-anchored monolith from a separate chip.
License
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Subject
Dichiarazione dei diritti
Publisher
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 8-bit Grayscale, 24-bit Color) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces

Le relazioni

Parents:

This work has no parents.

In Collection:

Elementi

Thumbnail Titolo Data caricata Visibilità Azioni
file details DoneanuAngela2005.pdf 2017-08-08 Pubblico Scaricare