Graduate Thesis Or Dissertation
 

Induction of hepatic microsomal enzymes in rainbow trout by dietary aroclor 1254 and the effect of cyclopropene fatty acids

Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/gq67jt86t

Descriptions

Attribute NameValues
Creator
Abstract
  • A dietary level of Aroclor 1254 (100 ppm) was fed to rainbow trout (Salmo gairdneri) for 15 weeks to determine the effects on hepatic microsomal enzyme induction. Fish were also fed combined polychlorinated biphenyl (PCB) (100 ppm) and cyclopropene fatty acids (CPFA) (50 ppm) to determine the effects on mixed function oxidase (MFO) induction. Dietary PCBs markedly induced the microsomal activities of 7-ethoxyresorufin O-deethylase, 7-ethoxy-coumarin O-deethylase, and benzo(a)-pyrene monooxygenase. Ethoxyresorufin O-deethylase activity continued to increase to a level 77-fold higher than control at week 15. Ethoxycoumarin O-deethylase and benzo(a)-pyrene monooxygenase activities increased to 7.1-fold and 48-fold over control at week 9 and then slightly decreased to 6.8-fold and 45-fold over control at week 15, respectively. Cytochrome P450 values remained approximately 2-fold above controls from week 5 through week 15. At weeks 1 and 3, cytochrome P450 levels were not significantly different from control. Ethoxyresorufin O-deethylase, ethoxycoumarin O-deethylase, and benzo(a)pyrene monooxygenase activities in the combined PCB and CPFA-fed trout were significantly higher than in controls and CPFA-fed fish, and significantly lower than in PCB-fed fish. There was no significant difference in cytochrome P450 levels after week 5. This is the first time dietary PCBs have been shown to induce the MFC system in PCB-fed rainbow trout.
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Publisher
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using Scamax Scan+ V.1.0.32.10766 on a Scanmax 412CD by InoTec inPDF format. LuraDocument PDF Compressor V.5.8.71.50 used for pdf compression and textual OCR.
Replaces

Relationships

Parents:

This work has no parents.

In Collection:

Items