Graduate Thesis Or Dissertation
 

Gene structure in Saccharomyces cerevisiae : DNase I hypersensitive sites in galactose gene cluster and non-detection of 5-methylc[yt]osine

Pubblico Deposited

Contenuto scaricabile

Scarica il pdf
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/gx41mm72z

Descriptions

Attribute NameValues
Creator
Abstract
  • The positions of the DNase I hypersensitive sites within the galactose gene cluster of the yeast Saccharomyces cerevisiaa have been determined. When yeast nuclei were incubated with DNase I, the purified DNA restriction endonuclease digested and analyzed by Southern hybridization, five hypersensitive regions were observed in this cluster of three closely spaced genes. Areas between the 5' end of GAL 7 and the 3' end of GAL 10 and between the two 5' ends of the divergently transcribed GAL 10 and GAL 1 genes contain DNase hypersensitive regions. DNase I hypersensitive sites were also observed in the areas near the 3' ends of the GAL 7 and GAL 1 genes. An additional site was observed within the GAL 10 structural gene. The region between GAL 10 and GAL 1 contains three tightly grouped cutting sites which map within a localized region of very high GC content. None of the five hypersensitive regions were observed when isolated DNA was digested with DNase I. Hypersensitive sites were present irrespective of the level of transcription of the galactose genes. A deletion which eliminates the 5' end of GAL 10 and all of GAL 1 does not destroy the internal GAL 10 hypersensitive site. The DNA of Saccharomyces cerevisiae has previously been reported to contain the modified base 5-methylcytosine. In order to determine if this methylation might occur in CCGG sequences, restriction endonuclease digestions were performed on DNA of strain B273-10B with the isoschizomer pair HpaII and Mspi. These two enzymes differ in their ability to cut the sequence C(5MeC)GG. Ethidium bromide stained gel tracks of DNA restricted with these two enzymes appeared identical. The patterns of HpaII and Mspl fragments within a 7.5 kb region about the gene for iso-2 cytochrome c and within the 6.3 kb two micron circle plasmid were also identical. Failing to have found any methylation in CCGG sequences, the genomic content of 5-methylcytosine was re-evaluated. HPLC analysis of highly purified yeast DNA showed 5-methylcytosine to be undetectable (less than 0.05% of total cytosine).
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Dichiarazione dei diritti
Publisher
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 24-bit Color) using Capture Perfect 3.0.82 on a Canon DR-9080C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces

Le relazioni

Parents:

This work has no parents.

In Collection:

Elementi

Thumbnail Titolo Data caricata Visibilità Azioni
file details ProffittJohnH1983.pdf 2017-08-10 Pubblico Scaricare