Graduate Thesis Or Dissertation
 

Identification of Milk-derived Peptides in Human Blood

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/h128np36p

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  • The digestive fate of milk proteins and peptides has been contested in the field of nutrition for decades. The objective of this research was to add clarity to this ongoing debate by 1. systematically reviewing studies found in Web of Science, PubMed and Google Scholar that found or searched for and did not find milk proteins or peptides in blood, 2. validating a method to identify milk peptides in human blood plasma if present and 3. analyzing plasma samples of human subjects after ingestion of a whey protein isolate (WPI) feed. From the literature review, 108 articles searched for milk proteins or peptides in blood. Of the 108, 102 detected absorbed proteins or peptides from either neonates or adults in human, monkey, pig, dog, rat, mouse, guinea pig, horse, goat, sheep, bovine, buffalo or fish subjects. For the second and third study aims, method validation was an important step to adequately identify dietary milk peptides among the complex blood matrix. After spiking an in vitro digest of glycomacropeptide (GMP) into blood, 43 GMP fragment peptides were extracted and detected. Using the same method to extract peptides from in vivo samples, milk-derived peptides were detected in only one out of 18 subjects after administration of WPI, many of which were homologous to bioactive sequences. Thus, future studies should consider the transfer of dietary peptides to the bloodstream and individual factors that may influence this process.
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