Molecular relationships among multiple-sized plasmids of Pseudomonas syringae pv. phaseolicola Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/h415pd863

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  • Five strains of the bean pathogen Pseudomonas syringae pv. phaseolicola were analyzed for plasmid number, size, and relatedness. Each strain contained one or two indigenous plasmids which ranged in size from 6.8 to 151 kbp. BamHI and EcoRI restriction patterns revealed that total plasmid DNA from each strain had a high level of sequence homology with pMC7105, a 148 kbp integrative plasmid found in a sixth strain. Solution hybridization confirmed these results; total plasmid DNA from each strain had 76 to 100 percent sequence homology with pPP6220, a plasmid essentially identical to pMC7105. The distribution of homology between pMC7105 and each plasmid was determined from filter hybridizations. Only six BamHI fragments from the plasmids in these strains failed to hybridize with pMC7105 probe. Four of these fragments, three from pPP6520 and one from pPP6525 of strain PP652, hybridized strongly to plasmid DNA from a closely-related pathovar, P. syringae pv. glycinea. BamHI fragment 8, which is involved in the integration of pMC7105 into the host chromosome, contains a repeat sequence that was present on all the plasmids except pPP6120 (6.8 kbp), pPP6310 (40 kbp), and pPP6520 (45 kbp). Every plasmid but pPP6520 had fragments that showed weak hybridization to the small plasmid, pPP6120. This homology suggests that a second repetitive sequence is common to these plasmids. The large plasmids (148 to 151 kbp) were essentially identical to pMC7105. The intermediate plasmids (122 to 128 kbp) appeared to be derived mainly from pMC7105 or a related plasmid, whereas the smaller plasmids (6.8 to 45 kbp) appear to have been derived in part from sequences not present in pMC7105.
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