Graduate Thesis Or Dissertation
 

Bioprotection of unseasoned Ponderosa pine lumber from microbial discoloration

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/hd76s210h

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  • The potential of 9 bacterial and 6 fungal isolates to protect wood against wood staining microorganisms was studied using small sapwood samples of unseasoned Ponderosa pine [Pinus ponderosa Laws] sapwood. Bacillus subtilis Cohn, isolate 733 A , Gliocladium virens J.H. Miller, J.E. Giddens & A.A. Foster isolate H 3 and Pseudomonas putida Migula isolate A-12 were identified as promising biological agents. The effect of media on the ability of B. subtilis # 733 A and B. subtilis ATCC 6633 to protect Ponderosa pine specimens from microbial discoloration was evaluated. A media preference was demonstrated with each of the species tested, which enhanced bioprotectant capability. B. subtilis # 733 A prevented fungal stain on Ponderosa pine to a greater degree than B. subtilis ATCC 6633. Extracts from Ponderosa pine specimens inoculated with B. subtilis # 733 A and/or fungal stain, exhibited no evidence of antibiosis against selected stain fungi and thus yielded little information on the modes of action of the biological agent. In situ observations on spore germination of Aspergillus niger van Tieghe isolate BOB in the presence of B. subtilis 733 A using a designed incubation chamber suggested that prior bacterial colonization of the wood surface did not inhibit germination of A. niger conidia. These results indicate that bioprotection is affected by a variety of factors including wood species, temperature, nutritional status, and isolate source. Considerable research will be necessary to successfully develop effective bioprotectants against fungal stain.
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