A chromosome analysis of five cell lines from four species of salmonid fishes Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/hd76s3021

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  • Four cell lines derived from embryonic tissues of four species of salmonid fishes, and one from a hepatoma in rainbow trout have been established in this laboratory. The species represented are chinook, coho, and sockeye salmon; and steelhead and rainbow trout. All of these lines have been cultivated for approximately three years. A chromosome analysis was performed on these cell lines and two primary cell cultures from embryonic tissues of chinook and coho salmon. Each analysis consisted of two parts, chromosome preparation and chromosome characterization. Chromosome preparation involved exposing a growing culture to colchicine. The cells were harvested, flattened, expanded, fixed, and stained. Chromosomes were characterized by selecting 100 metaphase cells from stained preparations of each cell culture, and determining the number of chromosomes per cell and each chromosome's morphology. At the time of analysis, the cell lines were about two years old, and the primary cultures were three to six weeks old. The modal numbers and ranges of chromosomes per cell for the five cell lines were as follows: chinook salmon line (TC-114), mode 71, range 18 to 190; coho salmon line (TC-119), mode 71, range 57 to 173; sockeye salmon line (SeE), mode 56, range 51 to 101; steelhead trout line (TC-137), mode 62, range 58 to 126; and rainbow trout line (TC-149), modes 54 and 60, range 18 to 144. Similar distributions for the two primary cell cultures were the following: chinook salmon culture, mode 68, range 35 to 172; and coho primary culture, mode 60, range 58 to 119. These data indicate that the chinook and coho salmon, and the steelhead and rainbow trout hepatoma lines were heteroploid. Also, the sockeye salmon line, and coho and chinook salmon primary cultures had normal diploid chromosome constitutions. All seven cell cultures showed ranges and/or distributions of chromosome numbers per cell that were greater than found in normal in vivo cells. Chromosome morphology was determined by classifying each counted chromosome as either metacentric or telocentric. The morphology of the chromosomes in each sample of 100 cells was represented by the average ratio of telocentrics to metacentrics per cell. The sockeye salmon and rainbow trout cell lines and the chinook primary cell culture had ratios similar to those reported for these species. The other cultures had ratios which differed slightly from those characteristic of the species.
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