Graduate Thesis Or Dissertation
 

Biosynthesis of streptomyces griseus phage deoxyribonucleic acid

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  • The presented study was undertaken to determine the operational catabolic carbohydrate pathway(s) contributing to the synthesis of the DNA of the actinophage for Streptomyces griseus. The incorporation of glucose-6-¹⁴C versus glucose-1-¹⁴C was determined for the deoxyribose and purine and pyrimidine bases of the phage DNA. The specific activities of the pentose and base moieties were calculated. Methods for the purification of the phage and isolation of the phage DNA were used. Development of deoxyribose isolation and quantitation procedures were of major importance in the investigation. The purine and pyrimidine bases were determined by standard methods. Radiotracer experiments were performed by liquid scintillation technique. It was concluded that the S. griseus phage DNA deoxyribose was synthesized primarily by the non-oxidative transketolase-transaldolase series of reactions. A minor deoxyribose synthesizing pathway was determined to be the oxidative hexosemonophosphate scheme. The phage DNA bases were not synthesized via a carbohydrate mechanism.
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