Feasibility of developing bread wheat cultivars with acceptable protein content for non-traditional areas Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/hq37vs009

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  • The objective of this investigation was to evaluate the possibility of developing high yielding wheat cultivars whose flour would result in acceptable loaf volumes for non-traditional bread wheat growing areas. Spring wheat germplasm employed included high protein hexaploid derivatives from Triticum dicoccoides, and selections from Argentina with good bread milling and baking properties. Winter wheat materials were from the Oregon State University International Winter x Spring Wheat Improvement Program. Elevated protein content was found not to be a prerequisite in obtaining high loaf volumes. Electrophoretic analysis (SDS-PAGE) of the high molecular weight glutenin subunits, and the sodium dodecyl sulphate sedimentation (SDSS) test proved to be reliable indicators of protein quality. High molecular weight glutenin banding patterns were independent of environmental factors. Bands 5-10 contributed by the D genome, with either bands 1 or 2* from the A genome and bands 7-8 or 17-18 coded by the B genome, appeared to be the most critical in influencing high loaf volume. For the experimental material used, SDSS values were found to be dependent upon variations in protein content. Five grams wholemeal flour samples with 2% SDS, 10 ml/I of 9.6% lactate solution and a reading time of 30 minutes provided the best combination to discriminate between lines with high or low loaf volume. Grain yield was found to be negatively associated with grain protein content in the spring wheat material. In crosses with Argentine lines, high protein "dicoccoides" derivatives, did not contribute major genes for protein content. Grain hardness was found to be a qualitatively inherited trait, and not associated with grain protein content or loaf volume. No single criterion proved of value in improving protein content and grain yield simultaneously, other than delaying selection until later generations. Strong negative associations between harvest index and grain protein content were observed for the winter wheat germplasm. Biological yield and kernel weight were the best selection criteria to improve grain protein without sacrificing grain yield. Early season applications of nitrate nitrogen resulted in a simultaneous increase in protein content and grain yield.
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