Evaluation of kasugamycin-use strategies designed to delay development of resistance in Erwinia amylovora Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/hx11xj12c

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  • The antibiotics, streptomycin sulfate and oxytetracycline, are used widely for fire blight suppression in the Pacific Northwest region of the United States. The efficacy of streptomycin, however, is compromised by widespread streptomycin-resistance in E. amylovora populations, and oxytetracycline is only partially effective. Consequently, for several years, the aminoglycoside antibiotic, kasugamycin (Kasumin 2L and 10L), was evaluated as an alternative material for fire blight control. Research focused on development of kasugamycin-use strategies with potential to delay the development of pathogen-resistance compared to use of kasugamycin alone. The first strategy involved use limitation through integration with biological control. Pantoea vagans was applied at 30 and 70% bloom followed by Kasumin at full bloom. Kasugamycin-resistant strain, P. vagans C9-1[superscript Kr], was obtained through step-wise selection from parental strain, P. vagans C9-1S. Comparison of establishment of these strains on flowers after overspray with Kasumin at 100 ppm showed C9-1[superscript Kr] with incidences of colonization averaging 69% and 63% on pear and apple, respectively, compared to 63% and 55% respectively, for C9-1S. Relative to the water-treated control, either strain followed by one application of Kasumin provided a similar level of blight control (84%), which was somewhat less than the 90-92% control observed after two applications of Kasumin at 100 ppm. There was no apparent benefit obtained from use of strain C9-1[superscript Kr] compared to strain C9-1S. The second resistance management strategy involved mixing Kasumin with oxytetracycline. In particular, a mixture of Kasumin at 80 ppm with oxytetracycline at 80 ppm was as effective as Kasumin at 100 ppm. In pear, an integrated treatment comprised of C9-1[superscript Kr] at 30 and 70% bloom followed by the 80 plus 80 ppm mixture of Kasumin and oxytetracycline resulted in 93% control. Kasugamycin-use strategies involving a) use limitation through integration with biological control, and b) rate reduction and mitigation of selection pressure through antibiotic mixtures each can achieve effective blight control, and may potentially slow the development of pathogen-resistance to this material.
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  • description.provenance : Rejected by Julie Kurtz(julie.kurtz@oregonstate.edu), reason: Rejecting to add at the bottom center of the title page - Commencement June 2012. Once revised, open the item that was rejected. Replace the attached file with the revised file and resubmit. Thanks, Julie on 2011-06-20T15:56:12Z (GMT)
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  • description.provenance : Approved for entry into archive by Julie Kurtz(julie.kurtz@oregonstate.edu) on 2011-06-22T15:43:23Z (GMT) No. of bitstreams: 3 license_rdf: 21627 bytes, checksum: ca446e9710dbec3bdf2cc67e04dc6cca (MD5) license_text: 21333 bytes, checksum: ffe7d4838c237b80bbcafd855eaeebbb (MD5) completed.pdf: 401420 bytes, checksum: 0942bdfee9b226821c056741b06a179c (MD5)
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  • description.provenance : Approved for entry into archive by Laura Wilson(laura.wilson@oregonstate.edu) on 2011-06-22T20:19:49Z (GMT) No. of bitstreams: 3 license_rdf: 21627 bytes, checksum: ca446e9710dbec3bdf2cc67e04dc6cca (MD5) license_text: 21333 bytes, checksum: ffe7d4838c237b80bbcafd855eaeebbb (MD5) completed.pdf: 401420 bytes, checksum: 0942bdfee9b226821c056741b06a179c (MD5)

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