Breeding tools for Cuphea : An updated genetic linkage map and fatty acid mutant candidate gene analysis Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/j098zd368

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  • Medium chain fatty acids (MCFAs) are important industrial feedstocks for soaps, detergents and surfactants. Interspecific lines derived from Cuphea lanceolata and Cup hea viscosissima are being domesticated as a temperate source of MCFAs. These species also serve as model organisms for understanding the biosynthesis of MCFAs in seeds. Various markers (176 SSRs, 22 fatty acid synthesis loci, and 27 converted RFLP markers) were screened for polymorphism among at least four genotypes (VS55, LN185, LN193-S1, and PSR23). The SSRs had heterozygosities ranging from 0.278 to 0.889. An F2 population ((LN185 x LNI193-S1, 91 individuals) of C. lanceolata (x=6) was genotyped at 90 loci for constructing a genetic linkage map. Eighty eight loci (48 SSRs, 23 SSCPs, 7 SNPs, 10 fatty acid synthesis loci) coalesced into eight linkage groups (two markers segregated independently). The map was 340.8 cM long with a mean density of 3.9 cM per locus. This linkage map will be a useful tool for the continued breeding and domestication of Cuphea.
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