The role of the homeodomain transcription factor Pitx2 in regulating skeletal muscle precursor migration and higher order muscle assembly Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/j098zf27h

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  • Cells of the ventrolateral dermomyotome delaminate and migrate into the limb buds where they give rise to all muscles of the limbs. The migratory cells proliferate and form myoblasts, which withdraw from the cell cycle to become terminally differentiated myocytes. The regulatory mechanisms that control the later steps of this myogenic program are not well understood. The homeodomain transcription factor Pitx2 is expressed specifically in the muscle lineage from the migration of precursors to adult muscle. Ablation of Pitx2 results in distortion, rather than loss, of limb muscle anlagen, suggesting that its function becomes critical during the colonization of, and/or fiber assembly in, the anlagen. Microarrays were used to identify changes in gene expression in flow-sorted migratory muscle precursors from Wild type and Pitx2 null mice. Changes in gene expression were observed in genes encoding cytoskeletal, adhesion and fusion proteins which play a role in cell motility and myoblast fusion. We observed decreased cellular motility, disrupted cytoskeleton organization and focal adhesion distribution, decreased fusion of mononucleated myoblasts into multinucleated myotubes and decreased proliferation in presence of Ptix2. These studies suggest that Pitx2 plays a critical role in regulating the timing of myoblast filling the limb anlagen which may have detrimental consequences for higher order muscle architecture.
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  • description.provenance : Rejected by Julie Kurtz(julie.kurtz@oregonstate.edu), reason: Rejecting to change the commencement date to June 2013. Once revised, open the item that was rejected. Replace the attached file with the revised file and resubmit. Thanks, Julie on 2012-06-22T21:27:16Z (GMT)
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  • description.provenance : Approved for entry into archive by Laura Wilson(laura.wilson@oregonstate.edu) on 2012-07-02T17:41:45Z (GMT) No. of bitstreams: 3 license_rdf: 21791 bytes, checksum: edec36a05ea144a7d0904ee6714c2151 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) campbelladam2013.pdf: 3122221 bytes, checksum: 7efe80b73bdd3c72a18bce1666c6e857 (MD5)
  • description.provenance : Submitted by Adam Campbell (campbada@onid.orst.edu) on 2012-06-22T00:14:41Z No. of bitstreams: 3 CampbellAdamL2012.pdf: 3121051 bytes, checksum: 32afba31e158464d0af5b0d11b87e569 (MD5) license_rdf: 21791 bytes, checksum: edec36a05ea144a7d0904ee6714c2151 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)
  • description.provenance : Approved for entry into archive by Julie Kurtz(julie.kurtz@oregonstate.edu) on 2012-06-25T20:12:13Z (GMT) No. of bitstreams: 3 campbelladam2013.pdf: 3122221 bytes, checksum: 7efe80b73bdd3c72a18bce1666c6e857 (MD5) license_rdf: 21791 bytes, checksum: edec36a05ea144a7d0904ee6714c2151 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)
  • description.provenance : Made available in DSpace on 2012-07-02T17:41:45Z (GMT). No. of bitstreams: 3 license_rdf: 21791 bytes, checksum: edec36a05ea144a7d0904ee6714c2151 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) campbelladam2013.pdf: 3122221 bytes, checksum: 7efe80b73bdd3c72a18bce1666c6e857 (MD5) Previous issue date: 2012-05-31

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