Graduate Thesis Or Dissertation

 

The antibody response of juvenile coho salmon (Oncorhynchus kisutch) to Aeromonas salmonicida, the causative agent of furunculosis Public Deposited

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  • This research was initiated to investigate the effects of various parameters on the immune response of juvenile coho salmon to Aeromonas salmonicida. The antibody response and development of protective immunity in juvenile coho salmon were followed after a single intraperitoneal injection of formalin killed A. salmonicida cells emulsified in Freund's (complete) adjuvant. The first elevated antibody levels and protective immunity were detected four weeks after injection. High antibody titers were observed for a year after injection. Animals as small as 1.2 g were immunologically competent to administration of this antigen preparation. In a representative experiment, 96.7 percent of the animals injected with this preparation produced high levels of antibody. Selected methods of infection with A, salmonicida were investigated. Variability of culture virulence was demonstrated by injected LD₅₀ values, which ranged from a single cell to 10⁸ cells. Endotoxin was extracted from A. salmonicida cells with trichloroacetic acid. This endotoxin was found to be non lethal, but was antigenic to juvenile coho salmon at doses ranging from 1 to 5, 000 μg when administered intraperitoneally with and without an aluminum hydroxide carrier. The A. salmonicida endotoxin was lethal to white mice when administered by this same route. LD₅₀ values for several preparations ranged from 232 to 536 μg per mouse. An antibody response to A. salmonicida endotoxin was demonstrated in juvenile coho salmon held at 6.7°C, 12.2°C and 17.8°C. At these temperatures the first elevated antibody levels were observed at four, two and one week, respectively.
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