Graduate Thesis Or Dissertation
 

Developmental axial skeletal deformities : baseline study with a zebrafish model

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/jq085p10b

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  • The aim of this study was to assess sensitivity of early life stage zebrafish to cadmium (Cd). Embryos and larva were exposed to Cd before the formation of skeletal elements. Exposure times were selected to determine whether Cd perturbed developmental processes that lead to skeletal deformities, and to characterize the types of skeletal deformities that occurred. Embryo/larva were exposed to 3μM, 10μM, 30μM, 100μM, 300μM, and 1000μM Cd at 12-36 hours post fertilization (hpf), 36-60hpf, 60-84hpf, and 144-168hpf in one series of experiments. This experiment was conducted at circumneutral pH. A second series of experiments with these same Cd concentrations were also conducted at pH 6 with embryo/larva exposed at 12-36hpf, 36-60hpf, and 60-84hpf. The fish that survived the exposures were raised for 40-60 days whereupon the surviving fish were overdosed with MS222, and X-rayed. The later early life stage fish were more sensitive to Cd toxicity; the chorion appeared to protect the earlier exposed embryos (zebrafish hatch 48-72hpf). Embryo/larva were more resistant to toxicity from dissolved Cd (pH 6) than particulate Cd (circumneutral pH); absence of functional gills during early life stages perhaps explained resistance to dissolved Cd. Notochord lesions (typically lethal within two weeks) occurred when embryos were exposed 12-36hpf. There was no evidence for sensitivity of early life stage zebrafish to Cd induced skeletal deformities that occurred within 40-60 days of Cd exposures.
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  • File scanned at 300 ppi (Monochrome, 256 Grayscale) using Capture Perfect 3.0.82 on a Canon DR-9080C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
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