X-ray structure studies of oriented phospholipid multilayers Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/k643b3693

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  • Lamellar x-ray diffraction was studied from thin films of L-α-1,2-dipalmitoyl lecithin (DPL) and a 1:1 mixture (BrDPL) of L-α-l-palmitoyl-2-(9- bromopaimitoyl) lecithin and its 10-bromo analog. The specimens had high lamellar order, with an upper bound on the mosaicity of 6 mrad (0.4°) for DPL and BrDPL. With various water contents, BrDPL underwent a broad, thermal transition centered around 0°C. The transition was evidenced by a reduction in lamellar spacing and a reduction in higher-order reflections at higher temperatures. In these respects, the transition was like the gel-liquid crystal transition known to occur in other phospholipids. An isomorphous replacement procedure was used to find the phase factors needed to synthesize electron-density profiles (assumed symmetric) for DPL and BrDPL. The resulting profile for DPL was similar to profiles which have been reported for phospholipid multilayers. The profile deduced for BrDPL was also similar, with the addition of a peak corresponding to the location expected for bromine if the hydrocarbon chains were fully extended. A one-dimensional, position-sensing proportional counter was built and characterized, in order to collect diffraction data from the lipid specimens. Using it, survey scans of the specimens were done in five minutes. Similar scans required several hours when film was used.
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