Characterization of acid sensing ion channel (ASIC) in mouse olfactory bulb Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/k930c076r

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  • This study examined the role of acid- sensing ion channels (ASICs) in interneuron synaptic modulation. ASICS are ultra fine discriminators of acidic conditions in the neuronal microenvironment. Each ASIC subunit isoform has innate, subtly different electrophysiological properties and tissue distributions. Structurally, ASICs may form homo- or heteromeric channels, yielding combinatorial functional properties regarding their sensitivity to the extracellular environment, saturation limits, inactivation profiles, and putative roles in the initiation of neuronal injury. Electrophysiology provides direct biophysical monitoring of channel activity and allows pharmacological isolation and manipulation. Fura-2 imaging allows direct visualization of calcium ion flux into neurons, verifying their calcium gating ability. Molecular verification of ASIC protein composition via western blotting and Immunocytochemistry showed anatomical distribution patterns of ASIC isoform combinations in the mouse olfactory bulb (OFB) tissue, the first documentation. Genetic knockout animals also verified results and internal controls were also utilized. ASIC isoforms combine their functions perform environmental sensing, and modulation of synaptic transmissions during normal and pathological conditions.
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  • description.provenance : Submitted by Linda Kathman (linda.kathman@oregonstate.edu) on 2008-02-04T17:48:44Z No. of bitstreams: 1 EricKratzer.pdf: 1316797 bytes, checksum: 79b244556edeb05f8075f10162922de9 (MD5)
  • description.provenance : Approved for entry into archive by Linda Kathman(linda.kathman@oregonstate.edu) on 2008-02-06T23:48:44Z (GMT) No. of bitstreams: 1 EricKratzer.pdf: 1316797 bytes, checksum: 79b244556edeb05f8075f10162922de9 (MD5)
  • description.provenance : Approved for entry into archive by Julie Kurtz(julie.kurtz@oregonstate.edu) on 2008-02-04T18:27:49Z (GMT) No. of bitstreams: 1 EricKratzer.pdf: 1316797 bytes, checksum: 79b244556edeb05f8075f10162922de9 (MD5)
  • description.provenance : Made available in DSpace on 2008-02-06T23:48:44Z (GMT). No. of bitstreams: 1 EricKratzer.pdf: 1316797 bytes, checksum: 79b244556edeb05f8075f10162922de9 (MD5)

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