Graduate Thesis Or Dissertation
 

Immunoelectrophoretic techniques used for systematic investigation

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/k930c104q

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  • Immunotaxonomic studies have largely been based on serology in which either one of two parameters has been used for establishing relationships: (1) the number of precipitin arcs formed on Ouehterlony plates, or (2) the total amount of precipitin resulting from reactions with a standard antiserum. Recognizing the restrictions of both methods, electrophoresis and immunology, it seems desirable to develop and evaluate techniques incorporating these primary tools as a means of determining protein homologies. Immunoelectrophoretic analysis (IEA) has special applicability for it provides a means of more objectively evaluating homologies at the generic and family levels. With the prime purpose of evaluating and developing techniques, two hypotheses are posed: (1) that homologous pairs of proteins are more common in related species and less common in more distantly related species, and (2) that proteins from distantly related organisms with common R[subscript f] values, when separated on acrylamide gels, can more likely be considered to be homologous if they exhibit the same antigenic properties. The purpose of this study was to determine the extent to which IEA can be used to establish isology and possibly homology among soluble insect proteins. Insect species whose relationships are well known have been used to determine whether IEA reflects on the propinquity of the species tested. The contention and conclusion of this investigation was that the techniques presented are of highly significant value in establishing phyletic relationships in closely and distantly related organisms.
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