Graduate Thesis Or Dissertation
 

Some physiological studies on cells of Vibrio marinus grown at 4 C and 15 C

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  • Cells of an obligately psychrophilic marine bacterium, Vibrio marinus, strain MP-1, were grown at 4 C and 15 C and studied for possible physiological differences. No differences were noted in sugar fermentation or temperature-shift growth studies; in both cases adequate nutrients were available. Some differences were noted in viability retention and manometric studies; adequate nutrients were not available. Fifteen C cells were more heat stable than 4 C cells. Fifteen C cells also utilized glucose at a higher rate than 4 C cells. These differences were attributed to differences in intra-cellular organization. The results indicated that in order to correlate laboratory results with in situ conditions, marine microorganisms should be studied when grown at the temperature of their natural habitat and without an abundance of nutrients.
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