Investigation of unique marine environments for microbial natural products Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/kh04ds000

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Metagenomics has revealed that the marine microbial biosphere is immensely more diverse than originally considered, and is an almost untapped reservoir for the potential discovery of microbial natural products. Despite numerous advances in culturing, biosynthetic engineering and genomic-based screening efforts to uncover much of this diversity in relatively accessible environments, a high rediscovery rate has resulted in the investigation of unique, relatively unexplored ecosystems harboring phylogenetically diverse communities of marine organisms. The focus of this research was to establish a culture repository of microorganisms collected from the Red Sea and from deep-sea hydrothermal vents, and to assess their biosynthetic potential for the production of new chemical scaffolds. Cultivation of marine cyanobacteria from the Red Sea has led to the identification of five new cyclic depsipeptides, apratoxin H, grassypeptolides D and E, Ibu-epidemethoxylyngbyastin 3 and leptochelin, the latter possessing a unique chemical scaffold capable of binding metals. A collection of deep-sea hydrothermal vent sediment and microbial mat samples led to the isolation of 64 unique bacterial strains, with eight assigned as members of the order Actinomycetales. Importantly, these isolates, along with a collection of deep-vent invertebrates and microbes, have led to the development of methods for the collection, culturing and biological screening of organisms from this extreme environment for future natural products research.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Keyword
Subject
Rights Statement
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Julie Kurtz(julie.kurtz@oregonstate.edu) on 2013-04-01T20:12:29Z (GMT) No. of bitstreams: 1 ThornburgChristopherC2013.pdf: 82323145 bytes, checksum: 4373a6948d0796bcd6c6b23c1ebdd489 (MD5)
  • description.provenance : Approved for entry into archive by Laura Wilson(laura.wilson@oregonstate.edu) on 2013-04-01T23:22:49Z (GMT) No. of bitstreams: 1 ThornburgChristopherC2013.pdf: 82323145 bytes, checksum: 4373a6948d0796bcd6c6b23c1ebdd489 (MD5)
  • description.provenance : Submitted by Christopher Thornburg (thornbuc@onid.orst.edu) on 2013-03-30T13:54:10Z No. of bitstreams: 1 ThornburgChristopherC2013.pdf: 82323145 bytes, checksum: 4373a6948d0796bcd6c6b23c1ebdd489 (MD5)
  • description.provenance : Made available in DSpace on 2013-04-01T23:22:49Z (GMT). No. of bitstreams: 1 ThornburgChristopherC2013.pdf: 82323145 bytes, checksum: 4373a6948d0796bcd6c6b23c1ebdd489 (MD5) Previous issue date: 2013-03-25

Relationships

In Administrative Set:
Last modified: 08/04/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items