Changes in the lipid composition of rainbow trout (Salmo gairdnerii) fed cyclopropenoid fatty acids Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/kk91fp872

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Rainbow trout were fed semi-purified diets containing cyclopropenoid fatty acids, and the biological effects of these acids were investigated. These fatty acids occur naturally in cottonseed oil and have been implicated in a large number of physiological disorders. Included in this study was an investigation of the pattern of deposition of cyclopropenes per se in trout tissue lipids, as well as the effect of these cyclopropenes on the deposition of other fatty acids. Other effects such as growth, liver size and histology were also noted. The ingestion of methyl sterculate, 2-octyl-1-cyclopropene-1- octanoic acid, by rainbow trout resulted in the deposition of intact cyclopropene in the body tissue. The accumulation in both the muscle and depot lipid reached a level approximately equivalent to that in the diet. This cyclopropene existed as the unaltered sterculate in the tissue triglycerides as shown by GLC analysis and was approximately equal to that predicted by the Halphen determination. A method was developed whereby trout liver lipid could be quantitatively analyzed for CPFA using a modified Halphen color reaction. This method was limited, however, by the small amounts of lipid normally available in trout liver. Ingestion of CPFA by rainbow trout resulted in reduction of growth. This effect was characterized by a large growth suppression early in the feeding period followed by a more normal pattern of gain. Dietary levels of 100 ppm and 200 ppm methyl sterculate resulted in identical effects. Livers from trout fed CPFA were typically enlarged and very firm as compared to normal trout livers. They also displayed a marked lack of pigmentation. Histological examination revealed extreme damage due to increased fatty infiltration and deposition. This phenomenon appeared to be most extreme during the early stages of feeding. Dietary cyclopropenoid fatty acids induced certain changes in the pattern of fatty acid deposition in rainbow trout. This alteration was characterized by a rapid increase in the concentration of stearic acid in the tissue, which resulted in a change in the ratio of stearic to oleic acid. A similar but less pronounced effect was also observed with palmitic acid and palmitoleic acid. The liver triglycerides of trout fed CPFA for an extended period also contained less 22:6w3 than normal. When these fish were fed a CPFA-free control diet their liver lipids returned to normal within 30 days.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 256 Grayscale) using Scamax Scan+ V.1.0.32.10766 on a Scanmax 412CD by InoTec in PDF format. LuraDocument PDF Compressor V.5.8.71.50 used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2012-01-18T16:38:39Z (GMT). No. of bitstreams: 1 ROEHMJEFFREY1968.pdf: 1091101 bytes, checksum: 2020f9e7f5aa60fc5d5efec9c744c605 (MD5) Previous issue date: 1968-02-09
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-01-12T18:59:23Z (GMT) No. of bitstreams: 1 ROEHMJEFFREY1968.pdf: 1091101 bytes, checksum: 2020f9e7f5aa60fc5d5efec9c744c605 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-01-18T16:38:39Z (GMT) No. of bitstreams: 1 ROEHMJEFFREY1968.pdf: 1091101 bytes, checksum: 2020f9e7f5aa60fc5d5efec9c744c605 (MD5)
  • description.provenance : Submitted by Erin Clark (ecscannerosu@gmail.com) on 2012-01-12T18:10:26Z No. of bitstreams: 1 ROEHMJEFFREY1968.pdf: 1091101 bytes, checksum: 2020f9e7f5aa60fc5d5efec9c744c605 (MD5)

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items