Characteristics of ice nucleation in potato leaves Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/kp78gj62h

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  • The innate ability of potato leaf tissue to initiate freezing was studied. Two potato species, the non-hardy cultivated potato Solanum tuberosum L. cv. Russet Burbank, and Solanum acaule Bitt. a hardy species from South America, were studied. Two treatments, low (cold) and high (warm) temperatures were included to study the effects of growing environment on ice nucleation activity. The nucleation characteristics of cell suspensions, leaf disks and leaflets were determined. A procedure was developed to isolate and purify mesophyll cell suspensions from potato leaves prior to testing of the cell suspensions. This procedure involved the use of clear adhesive tape attached to the upper and lower epidermis during enzymatic degradation. The tape held the epidermis together allowing its removal and exclusion from the resulting suspension. Mesophyll cell suspensions prepared in this way contained less than 11% epidermal cells. Data from droplet freezing assays of suspensions and leaf disks were plotted as percent frozen vs. temperature. No substantial nucleation activity occurred above -9°C. Nucleation activity for suspensions was lower than leaf disks. The temperature of ice formation in leaflets was determined by differential thermal analysis (DTA). Mean temperatures of freezing were -11.1 and -12.1°C for S. acaule warm and cold respectively, and -11.4 and -9.9°C for S. tuberosum warm and cold. There are no significant differences between means at the 5% level. Plant hardiness was determined by whole plant freezing. Cold grown S. acaule survived -6°C while warm grown S. acaule survived -4°C. S. tuberosum only tolerated -2°C for the warm and -2.5°C after a cold treatment. The differences between plant hardiness and leaf nucleation temperatures indicate that intrinsic leaf ice nucleators do not substantially limit supercooling in potato.
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