Graduate Thesis Or Dissertation
 

Micropropagation of Pyrus rootstocks

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  • The availability of new pear rootstocks could be increased by development of an efficient micropropagation system. Many P. calleryana, P. betulifolia and P. communis rootstocks are resistant to many pear diseases and are graft compatible with leading cultivars. Explains of three potentially useful rootstock selections P. calleryana Decne 'OPR 157', P. betulifolia Bunge 'OPR 260', and P. communis L. 'Old Home x Farmingdale 230' were initiated from forced branches of field-grown trees. Combinations of N⁶-benzyladenine (BA) at 0, 2, 4, 8 and 13 μM and Napthaleneacetic acid (NAA) at 0, 0.5, 1, 2 and 4 μM were tested on Woody Plant Medium (WPM) and Cheng medium and BA with Indole-3-butyric acid (IBA) on Cheng for shoot multiplication and elongation. Rooting was induced with NAA and IBA. Increased shoot proliferation was observed for all three genotypes with BA concentration being the most influential factor. For OPR 260 Cheng medium with IBA produced more shoots than Cheng with NAA and increased BA concentrations improved multiplication for either auxin. Shoot proliferation was higher for shoots grown on WPM with NAA than on Cheng with NAA and multiplication rates were lower for plants on media with NAA than with IBA. NAA concentration greater than 0.5 μM were inhibitory to shoot multiplication. Overall, the best micropropagation medium for OPR 260 was Cheng medium with 8 μM BA and 0.5 μM IBA. For OHxF 230 NAA at 0.5 μM and IBA at 0.5 and 1 μM were stimulatory to shoot multiplication but higher levels were inhibitory. Shoots on Cheng medium proliferated better than shoots on WPM. The best medium for micropropagation was Cheng medium with 8 μM BA and 0.5 μM IBA. Shoot multiplication of OPR 157 was better on NAA than IBA and lower auxin concentrations (0, 0.5 μM) were best with higher levels inhibiting multiplication. Shoots on either Cheng or WPM with NAA produced high multiplication rates and shoot proliferation increased with increasing BA concentrations up to 8 μM. Based on shoot proliferation and plant appearance, OPR 157 was best micropropagated on Cheng or WPM medium with 8 μM BA and 0.5 μM NAA. Rooting (>80%) was easily achieved for OHxF 230 with all IBA and NAA treatments. The best rooting (42.9%) for OPR 260 was on Cheng medium with 10 μM IBA (dark) and for OPR 157 (23.9%) was with a 15 sec dip in 10 μM NAA. All rooted plantlets survived four weeks of greenhouse acclimatization.
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file details YEODENNIS1994.pdf 2017-08-02 Pubblico Scaricare