Graduate Thesis Or Dissertation

 

Patterns of growth and seasonal changes in the concentrations of abscisic acid and indoleacetic acid in roots of western hemlock Public Deposited

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  • The capacity for root growth and concentration of indole-3-acetic acid and abscisic acid in the roots of western hemlock seedlings was investigated. The root regenerating capacity (RRC) was defined as the increase in root length, greater than 5 mm, following a 28-day test period. RRC measurements over a two-year period reveal a low potential for root growth in the late fall increasing to a maximum in late winter. The RRC remained high through the early spring after which it declined throughout the summer and fall. Seedling survival followed a similar trenth Cold storage did not appear to affect the RRC in nursery grown bare-root seedlings. Test environments in which soil temperature or light intensity was varied were used to investigate the possible mechanisms controlling root growth. Growth of western hemlock roots appears to be optimum at a soil temperature of 20°C. Root growth seems to decrease with increasing light intensity and is not adversely affected by low light intensities. The roots of western hemlock appear more sensitive to seedling disturbance in the fall than in the winter or spring. Isolation and quantitative analysis of indole-3-acetic acid (IAA) and abs cisic acid (ABA) from the roots of western hemlock were accomplished using gas-liquid chromatography. It appears that a high ABA concentration in the fall may be inhibiroty to root growth while a high IAA/ABA ratio during winter may account for the increased capacity for root growth during this period. An increase in ABA through the spring and summer may result in the decline in the RRC that is observed at this time.
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