Molecular characterization of crustacean parasite Nadelspora canceri Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/mc87pt52b

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Investigations into the phylogeny, genome size, and karyotype of microsporidian Nadeispora canceri were initiated to further characterize the organism. Isolates of N. canceri spores were obtained from both Dungeness (Cancer magisrer) and red rock crabs (Cancer productus). Analysis of the ssu rDNA sequence from spore isolates of the two crab species showed 100% sequence identity among 1,081 nucleotide positions, indicating the same species of microsporidian is infecting both species of crabs. Phylogenetic studies based on the ssu rDNA sequences also showed N. canceri to be most closely related to another crustacean parasite, A meson michaelis. Sequence comparison between the two microsporidian species showed 93% sequence identity (1,001/1081 nucleotide positions). Pulsed field gel electrophoresis was used to estimate the genome size and karyotype of N. canceri isolates obtained from Dungeness and red rock crabs. Resolution of DNA bands on the pulsed field gels revealed both isolates to have a karyotype often chromosome-sized DNA bands. Estimation of the genome size revealed spore isolates from C. magister to have a total genome size of 7.44 Mb and spore isolates from C. productus to have a total genome size of 7.32 Mb. Variations detected in chromosome size culminated in a difference in the genome size between the two isolates. However, the variations in chromosome size were found not to be significant based on the Student's t-test.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 8 bit grayscale) using ScandAll PRO 1.8.1 on a Fi-6770A in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-06-14T18:56:18Z (GMT) No. of bitstreams: 1 AmoganHarold2004.pdf: 3559047 bytes, checksum: 119f6522dc4ee53952150354da8719d3 (MD5)
  • description.provenance : Rejected by Patricia Black(patricia.black@oregonstate.edu), reason: replace on 2012-06-14T17:41:04Z (GMT)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-06-14T18:17:35Z (GMT) No. of bitstreams: 1 AmoganHarold2004.pdf: 3559047 bytes, checksum: 119f6522dc4ee53952150354da8719d3 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-06-13T16:24:50Z (GMT) No. of bitstreams: 1 AmoganHarold2004.pdf: 777762 bytes, checksum: d218c3c033caea98a959d39714c223c2 (MD5)
  • description.provenance : Submitted by Sergio Trujillo (jstscanner@gmail.com) on 2012-06-12T00:58:15Z No. of bitstreams: 1 AmoganHarold2004.pdf: 777762 bytes, checksum: d218c3c033caea98a959d39714c223c2 (MD5)
  • description.provenance : Submitted by Sergio Trujillo (jstscanner@gmail.com) on 2012-06-14T17:46:36Z No. of bitstreams: 1 AmoganHarold2004.pdf: 3559047 bytes, checksum: 119f6522dc4ee53952150354da8719d3 (MD5)
  • description.provenance : Made available in DSpace on 2012-06-14T18:56:18Z (GMT). No. of bitstreams: 1 AmoganHarold2004.pdf: 3559047 bytes, checksum: 119f6522dc4ee53952150354da8719d3 (MD5) Previous issue date: 2004-02-26

Relationships

In Administrative Set:
Last modified: 08/15/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items