Retention of wheat alleles in imidazolinone-resistant wheat x jointed goatgrass recurrent backcross generations Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/mk61rk87q

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  • The retention of D genome wheat alleles in the progeny of imidazolinoneresistant wheat (R-wheat) and jointed goatgrass hybrids backcrossed to jointed goatgrass was documented in this study. Resistance to the imazamox application was used as a marker for the imidazolinone-resistance gene. Fourteen simple sequence repeat (SSR) markers, for the wheat D genome, were used as unselected markers. R-wheat x jointed goatgrass F₁ hybrids were backcrossed to jointed goatgrass. The resulting BC₁ seed was divided into 2 sub-populations, BC₁s and BC₁u. The BC₁s population was treated imazamox at 26.9 kg ai/ha. The BC₁s survivors (five out of 10 plants) and the BC₁u subpopulation were backcrossed to jointed goatgrass to produce the BC₂s and BC₂u generations. The BC₂s population was treated with imazamox, and three out of 14 plants were resistant. The BC₂u sub-population was either self-pollinated to produce BC₂S₁ plants, or backcrossed to jointed goatgrass. The BC₂s plants produced no progeny. The BC₂S₁u and BC₃u plants were treated with imazamox. There were resistant individuals in the progeny groups of 10 of 12 BC₁s; 6 progeny groups would have been expected to contain resistant individuals. The D genome wheat SSR markers used in this study allowed polymorphism to be indentified between wheat and jointed goatgrass in 3% agarose gels stained with etbidium bromide. The SSR markers were inherited Mendelianly. The wheat SSRs, because they amplify polymorphic products from various loci in different sizes in wheat and jointed goatgrass will be a valuable tool to further examine gene flow between wheat and jointed goatgrass.
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