The genetic diversity of Ceanothus-infective Frankia Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/mk61rm37q

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Frankia from root nodules of nine different species of Ceanothus were characterized. DNA was amplified directly from nodular material using the polymerase chain reaction (PCR). The amplified region includes the 3' end of the 16S rRNA gene, the intergenic spacer (IGS), and a large portion of the 23S rRNA gene. Restriction enzyme digestions of PCR products allowed us to designate PCR-RFLP groups among the Ceanothus-infective Frankia tested. The groupings did not follow the taxonomic lines of the Ceanothus host species. Instead, the Frankia strains present followed a geographical pattern. This information was used to choose representative Ceanothus-infective Frankia for phylogenetic analysis. Full-length 16S rDNA sequences were amplified directly from the nodules of two Ceanothus species using the PCR. Sequences were determined using an automated sequencer, compared against GenBank, and assembled into consensus sequences. The sequences were aligned with other full-length Frankia 16S rDNA sequences available from the database. Phylogenetic trees were obtained from three different algorithms: neighbor joining, parsimony, and the maximum-likelihood method. These Ceanothus microsymbionts appear to be most closely related to the microsymbiont associated with Dryas drummondii using all three methods.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 256 Grayscale) using Capture Perfect 3.0 on a Canon DR-9050C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-09-21T20:07:33Z (GMT) No. of bitstreams: 1 RitchieNancyJ1998.pdf: 3094590 bytes, checksum: 896323fa5a0e19f127d676272fd423e7 (MD5)
  • description.provenance : Made available in DSpace on 2012-09-21T20:07:33Z (GMT). No. of bitstreams: 1 RitchieNancyJ1998.pdf: 3094590 bytes, checksum: 896323fa5a0e19f127d676272fd423e7 (MD5) Previous issue date: 1997-12-12
  • description.provenance : Submitted by Kirsten Clark (kcscannerosu@gmail.com) on 2012-09-20T20:10:03Z No. of bitstreams: 1 RitchieNancyJ1998.pdf: 3094590 bytes, checksum: 896323fa5a0e19f127d676272fd423e7 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-09-21T20:05:27Z (GMT) No. of bitstreams: 1 RitchieNancyJ1998.pdf: 3094590 bytes, checksum: 896323fa5a0e19f127d676272fd423e7 (MD5)

Relationships

In Administrative Set:
Last modified: 08/20/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items