Graduate Thesis Or Dissertation
 

Phospholipids in germinating Douglas fir seed

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  • Information concerning the kinds and composition of phospholipids in gymnosperm plants is negligible in the literature. Thus this study was undertaken to provide background knowledge for future comparative biochemical investigations. In this study, lipid was extracted by chloroform and methanol, and washed with distilled water to prevent the possible formation of phospholipid salts. Then, thin-layer, column and gas-liquid chromatographies were used for isolation of the phospholipids, separation of phospholipids into classes and analysis of fatty acids. Characterization of the different kinds of phospholipids was mainly by reagent spray and mild alkaline hydrolysis followed by identification with paper chromatography of the deacylated phospholipids. The total lipid content in endosperm and seedling of germinating Douglas fir seed was 40.5 and 17.5 percent (dry weight basis) respectively. The phospholipid contents were 3.6 and 22.6 percent of the total lipid respectively in the two kinds of tissues. The composition of phospholipids in the two tissues varied. "Phosphatidic acid mixture" was the major component in both tissues representing 37.6 percent of lipid phosphorus in the endosperm and 30.1 percent in the seedling. Phosphatidyl choline comprised 30.5 percent of lipid phosphorus in the endosperm and 25.7 percent in the seedling, whereas phosphatidyl ethanolamine was 13.2 percent and 21.8 percent, phosphatidyl serine 14.7 percent and 10.3 percent, and amino acid containing phospholipids four percent and 12.2 percent respectively for these two tissues.
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