Graduate Thesis Or Dissertation
 

Confidence limits for fluoresence tests of ryegrass (Lolium sp.)

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  • Studies were conducted to determine the variability existing in repeated fluorescence tests of annual and perennial ryegrass (Lolium multiflorum and L. perenne, respectively). Sources of variation considered were within-sample error, among-sample error, among-germinator error, and among-laboratory error. These were studied independently and the variation in each was compared to the theoretical variability due to random sampling error alone. Variability indices were calculated which showed the amount of experimental error observed in fluorescence test results due to each source of variation. These indices showed that the present fluorescence tolerances, based on random sampling from a binomial population and used by the Association of Official Seed Analysts are not adequate to account for the variability actually existing in repeated fluorescence test results conducted on the same sample in the same laboratory. When other sources of variation are introduced, the inadequacy of the present tolerances becomes even more pronounced. The differences between fluorescence tolerances used by the Association of Official Seed Analysts and the International Seed Testing Association are discussed and a new method of calculating fluorescence tolerances is presented.
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