Graduate Thesis Or Dissertation
 

Evaluation of effectiveness of Rhizobium trifolii strains for Trifolium subterraneum L. (subterranean clover) in southwestern Oregon soils

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/n870zt41z

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  • Twelve high effective Rhizobium trifolii strains were evaluated at four locations in southwest Oregon for the ability to effectively nodulate Mount Barker subterranean clover. All twelve strains possessed antibiotic resistance markers to facilitate identification of strains when extracted from nodules. No significant differences in plant dry weight means could be observed at two of the sites, while at the remaining two sites, plant dry weight means were significantly different. Nodule occupancy means averaged over these two sites were higher than those nodule occupancy means for the sites with no significant differences in plant dry weights. High variability was demonstrated between percent nodule occupancy and mean dry plant weight, and some possible explanations for these results are offered, including a discussion of the possible role of native strains. Total plant nitrogen values were found to be significantly different for all sites and the lowest nitrogen values were found in the site with the greatest plant dry weights. No one strain of Rhizobium trifolii could be demonstrated to perform significantly better than all other strains at all sites, and strains isolated in Oregon appeared to have no significant competitive or effective advantage over strains isolated outside of Oregon. The apparent enhancement of seed survivability by planting under the soil surface rather than broadcasting is mentioned.
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