Polyion sensors based on induced ion extraction Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/ng451m47h

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  • A novel method for detecting the peptidase activity of clinically relevant peptidases was investigated using solid-contact electrochemical polyion sensors under chronopotentiometric control. Complete instrumental control over ion-extraction was accomplished by incorporating a lipophilic electrolyte into the ion-selective membrane; the sensors had no intrinsic ion-exchange capabilities. The sensors were used to monitor the digestion of the polypeptide protamine by the peptidase trypsin. A linear relationship was observed between the initial rate of reaction and the amount of trypsin used in the digestion. In addition, the sensor’s response towards the products of the tryptic digestion was investigated. In a separate experiment, a synthetic peptide was synthesized containing specific cleavage sites for the peptidase chymotrypsin. Again, a linear relationship was observed between the initial rate of reaction and the amount of chymotrypsin used in the digestion. This demonstrated it was possible to design peptides specifically for the determination of a specific peptidase activity if the lipophilic electrolyte’s ability to stabilize peptides in the membrane is well understood.
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  • description.provenance : Approved for entry into archive by Linda Kathman(linda.kathman@oregonstate.edu) on 2009-06-24T23:18:26Z (GMT) No. of bitstreams: 1 Thesis, Final Copy.pdf: 1441744 bytes, checksum: aafa34bc3053ae8950e7c9754d88b615 (MD5)
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  • description.provenance : Approved for entry into archive by Julie Kurtz(julie.kurtz@oregonstate.edu) on 2009-06-23T16:11:14Z (GMT) No. of bitstreams: 1 Thesis, Final Copy.pdf: 1441744 bytes, checksum: aafa34bc3053ae8950e7c9754d88b615 (MD5)
  • description.provenance : Submitted by Katherine Fordyce (fordycek@onid.orst.edu) on 2009-06-18T21:45:27Z No. of bitstreams: 1 Thesis, Final Copy.pdf: 1441744 bytes, checksum: aafa34bc3053ae8950e7c9754d88b615 (MD5)

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