Graduate Thesis Or Dissertation
 

Fractionation of bovine muscle proteins by cellulose ion exchange chromatography

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  • The purposes for which the fractionation of proteins are carried out are quite varied and manyfold. However, one of the more important reasons is that of determining the nature and the extent of autolysis or proteolysis on the bovine muscle proteins during post mortem aging. Hence, the development of a procedure for the adequate fractionation of muscle proteins would greatly stimulate the interest and research progress in this difficult field of study. The research reported herein pertains to a study of the fractionation of fresh bovine muscle proteins by ion exchange chromatography. A KCl-phosphate buffer, pH 7.5 and an ionic strength of 0.55, was used to extract the proteins from the muscle. This extract was then diluted to specific ionic strengths in order to separate the gross fractions (actomyosin, myosin, sarcoplasmic + actin) from the total KCl-phosphate soluble proteins. The major protein fractions were then separated by diethylaminoethyl-cellulose (DEAE-cellulose) ion exchange chromatographic procedure. A non-linear gradient elution schedule was used throughout the chromatographic procedure. The disc electrophoresis technique was used to determine the homogeneity of the various protein fractions and sub-fractions. The total KCl-phosphate soluble proteins were fractionated into 9-12 fractions by DEAE-cellulose ion exchange chromatography. The number of fractions differed from one sample to another. The disc electrophoresis results paralleled those of the chromatographic procedure. Some of the fractions appeared to be homogeneous while others were not. The KCl-phosphate soluble proteins minus actomyosin were fractionated by column chromatography into 9-10 different protein components. The disc electrophoresis results also indicated that this fraction contained 9-10 components. The sarcoplasmic + actin proteins were fractionated into six fractions by the chromatographic procedure. These fractions appeared to be electrophoretically homogeneous. Although some success was attained in the separation of the actomyosin proteins, the myosin fraction was quite resistant to chromatographic separation. In spite of the ineffectiveness of the DEAE-cellulose column chromatographic technique to fractionate the myosin and actomyosin proteins, the procedure appears to have some value for studying the other protein fractions during autolysis. Moreover, further work on the adoption of this procedure might provide the necessary information to perfect the technique for muscle protein fractionation.
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file details MOHASSEBZEINAB1963.pdf 2017-08-15 Pubblico Scaricare