Naphthalene resistance in a house fly strain : its inheritance and characteristics Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/ns064966q

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  • A combination of genetical and biochemical methods were utilized in investigating house fly resistance to naphthalene. The inheritance of resistance was determined by crossing a naphthalene resistant strain of Musca domestica L. with a susceptible mutant marker strain. Biochemical comparisons were made on substrains isolated from the progeny of these crosses. Factors on chromosomes II, III and V were found to be important in resistance when F₂ and backcross progeny were bioassayed with naphthalene vapors. Statistical analysis of the bioassay results indicated that resistance factors on chromosome III were inherited as recessive or incompletely recessive genes, and the factors on chromosomes II and V were of a dominant nature. The factors on chromosomes II and III were most important in resistance. Toxicological experiments were conducted on the parent strains and the naphthalene tolerant substrains. In in vivo experiments with these strains, flies with chromosome III from the resistant parent were two to three times more resistant to knockdown by dieldrin, naphthalene, and tributyltin chloride than flies with susceptible alleles on this chromosome. Slow absorption of the toxicants is thought to account for this knockdown resistance. The oxidative activity of microsomal enzymes was found to be greater in substrains with chromosome II from the resistant parent. Both hydroxylation and epoxidation reactions were measured in these in vitro experiments, using naphthalene and aldrin as substrates. Aldrin epoxidation was more closely aligned with resistance in parent and substrains than naphthalene hydroxylation. Resistance due to factors on chromosome V could not be attributed to increased oxidase activity or to the slow absorption of toxicants. When the metabolic and non-metabolic factors were combined in a strain, resistance increased nearly three-fold. This complementary effect suggests that slow absorption (chromosome III) and active detoxication of naphthalene (chromosome II) interact, and allow flies to resist larger doses of naphthalene. Resistance due to factors on chromosomes II and III did not account for all of the resistance of the naphthalene strain, indicating that chromosome V probably plays a more important role when combined with other resistance factors.
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