Circular dichroism of amylose and glucose oligosaccharides in the vacuum ultraviolet Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/p2676z68w

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • The circular dichroism spectra of amylose, the maltose oligomers, cellobiose, and the cycloamyloses are measured in aqueous solution to 1640 A. Two bands are found in this region. The long wavelength band is assigned to the ether chromophores; the short wavelength band is assigned to the hydroxyls and further ether transitions. An examination of chromophorically equivalent but conformationally different glucans demonstrates that the circular dichroism of glucans is sensitive to conformation. However, the conformation of the subunits in the maltose oligomer series is independent of chainlength. The conformation of the interior maltosyl groups of this series are all approximately equivalent, and the conformation of the end groups are the same for each oligomer and only one glucosyl subunit in length. A comparison of the amylose and cycloamylose spectra indicates that amylose is substantially biased in its chirality. Since there is no chain length dependence, the oligomers must have the same chirality bias. The data of this study is consistent with conformational calculations on amylose which predict that the maltosyl subunits of amylose are restricted to a small region on its conformational map which includes the V form helix. The butanol complex of amylose, which is believed to have a V form helical conformation, has a circular dichroism similar to free aqueous amylose, indicating that maltosyl subunits of amylose are approximately equivalent for these two species. However, since heated amylose is precluded from having a helical conformation, the temperature insensitivity of the free amylose spectra shows that the maltosyl subunits of amylose are not identically V form. This is consistent with a model for amylose which forms a loose extended helix.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Submitted by Madison Medley (mmscannerosu@gmail.com) on 2013-10-24T21:47:24Z No. of bitstreams: 1 LewisDonaldG1977.pdf: 786005 bytes, checksum: df027fc4ab10c68737f32a8385097b4c (MD5)
  • description.provenance : Made available in DSpace on 2013-10-25T19:58:50Z (GMT). No. of bitstreams: 1 LewisDonaldG1977.pdf: 786005 bytes, checksum: df027fc4ab10c68737f32a8385097b4c (MD5) Previous issue date: 1976-05-27
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-10-25T14:33:22Z (GMT) No. of bitstreams: 1 LewisDonaldG1977.pdf: 786005 bytes, checksum: df027fc4ab10c68737f32a8385097b4c (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-10-25T19:58:50Z (GMT) No. of bitstreams: 1 LewisDonaldG1977.pdf: 786005 bytes, checksum: df027fc4ab10c68737f32a8385097b4c (MD5)

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items