Genetic components of variation in a bay mussel embryo bioassay Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/p5547t874

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  • The contribution of genetic effects to the variation in percent normal development observed in a bay mussel (Mytilus edulis L.) embryo bioassay was determined. A factorial breeding experiment was accomplished in which 9 males were mated with 3 females. Each mating was repeated 10 times, yielding 270 mussel embryo cultures, which were exposed to 0.00085 parts per million of mercury. This concentration was previously determined to be the level causing approximately 50 percent abnormal development in the progeny of the mussels used in the experiments. All matings were replicated in untreated sea water providing 270 control cultures. The treatment replicate and the control replicate represented two separate environments; therefore, a 3-factor analysis of variance was used to estimate the variance associated with male and female parents, and replications. The range of percent norm3l development for the non-treated cultures was 1.6 to 86.4 percent with a mean of 33.9 percent. The percent normal in the treated cultures ranged from 9.2 to 70.5 percent with a mean of 40.6 percent. The sources of variation related to genetic effects and a genotype-environment interaction were found to be significant. The implications of the results for the interpretation of bioassay results is discussed.
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