Detection, identification, and antibiotic treatments of bacterial contaminants from micropropagated strawberries Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/p5547v420

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Bacterial contamination is one of the most important problems both in plant tissue culture research and commercial laboratories. Early detection of contaminants saves time, effort, and money. Strawberry runner explants were screened for contaminants during June to August, 1994 and 1995. Most contaminants found were bacterial rather than fungal. Bacterial contaminants from 22 contaminated genotypes were isolated, purified, and identified to genus using standard biochemical tests such as Gram's stain, King's B medium, starch hydrolysis, and carbon source utilization (Biolog). From those 22 genotypes, 16 different bacterial strains were isolated. The majority were Gram-negative, motile, rod-shaped fluorescent pseudomonads. Xanthomonads, enterobacterium, and other unidentified Gram-negative and Gram-positive bacteria were also found. Minimal bactericidal concentrations (MBCs) of single (Timentin, streptomycin sulfate, dihydrostreptomycin, and gentamicin) and combinations of two and three (Timentin, streptomycin sulfate, and gentamicin) were tested for inhibition of the bacterial isolates. Combinations of Timentin, streptomycin sulfate, and gentamicin killed all bacterial isolates tested. Fragaria virginiana subsp. glauca (S. Watson) Staudt and F. x ananassa Duch. Cv. Jucunda were inoculated with Pseudomonas corrugata or Xanthomonas campestris pv. vesicatoria then treated with a combination of Timentin, streptomycin sulfate, and gentamicin. F. virginiana subsp. glauca died after inoculation with either bacteria. Antibiotic treatments were 100% effective in eliminating P. corrugata from 'Jucunda', but only 23% of the plants inoculated with X. campestris pv. vesicatoria were freed of the bacteria.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using Scamax Scan+ V.1.0.32.10766 on a Scanmax 412CD by InoTec in PDF format. LuraDocument PDF Compressor V.5.8.71.50 used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2011-11-22T17:51:14Z (GMT). No. of bitstreams: 1 TANPRASERTPIYARAK1996.pdf: 1116078 bytes, checksum: c2b623e0440a2927c9bc58e4e4247d88 (MD5) Previous issue date: 1996-05-13
  • description.provenance : Submitted by Erin Clark (ecscannerosu@gmail.com) on 2011-11-10T17:31:47Z No. of bitstreams: 1 TANPRASERTPIYARAK1996.pdf: 1116078 bytes, checksum: c2b623e0440a2927c9bc58e4e4247d88 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2011-11-10T23:09:48Z (GMT) No. of bitstreams: 1 TANPRASERTPIYARAK1996.pdf: 1116078 bytes, checksum: c2b623e0440a2927c9bc58e4e4247d88 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2011-11-22T17:51:14Z (GMT) No. of bitstreams: 1 TANPRASERTPIYARAK1996.pdf: 1116078 bytes, checksum: c2b623e0440a2927c9bc58e4e4247d88 (MD5)

Relationships

In Administrative Set:
Last modified: 08/15/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items