Isolation and partial characterization of a natural antioxidant from shrimp (Pandalus jordani) Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/p5547w44r

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  • The nature of a natural antioxidant present in shrimp was investigated. Shrimp extracts were prepared using different solvents to ascertain the best extraction method. The antioxidant activity was determined using the (β-carotene-linoleate model system and the oxygen weighing method. Ethanol proved to be the best solvent for extracting the natural antioxidant. Different shrimp materials extracted with ethanol proved to be equally effective as antioxidant sources. The non-lipid nature of the antioxidant was established by separating the lipid from the non-lipid material and measuring its antioxidant activity. The phenolic nature of the antioxidant was further established and its isolation achieved by means of preparative thin layer chromatography. The Rf values with different solvents and its wavelength of absorption maxima were determined. Its chemical nature was further investigated using different visualizing reagents. The colorimetric quantitation of the antioxidant factor revealed that it is present in very low concentrations in its natural source. Its effectiveness as an antioxidant at low concentrations together with the fact that it is a natural product makes it a valuable compound of potential use in the food industry.
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  • File scanned at 300 ppi (Monochrome) using Scamax Scan+ V.1.0.32.10766 on a Scanmax 412CD by InoTec in PDF format. LuraDocument PDF Compressor V.5.8.71.50 used for pdf compression and textual OCR.
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  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-02-01T22:14:22Z (GMT) No. of bitstreams: 1 PASQUELLORENZO1978.pdf: 292836 bytes, checksum: 80cf6fed9f6636403308490490692f45 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2011-12-12T17:08:25Z (GMT) No. of bitstreams: 1 PASQUELLORENZO1978.pdf: 292836 bytes, checksum: 80cf6fed9f6636403308490490692f45 (MD5)
  • description.provenance : Submitted by Erin Clark (ecscannerosu@gmail.com) on 2011-12-12T15:55:16Z No. of bitstreams: 1 PASQUELLORENZO1978.pdf: 292836 bytes, checksum: 80cf6fed9f6636403308490490692f45 (MD5)
  • description.provenance : Made available in DSpace on 2012-02-01T22:14:22Z (GMT). No. of bitstreams: 1 PASQUELLORENZO1978.pdf: 292836 bytes, checksum: 80cf6fed9f6636403308490490692f45 (MD5) Previous issue date: 1977-12-02

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