RFLP analyses of interspecific hybrids of Phaseolus Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/pc289m265

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  • Gene transfer via interspecific hybridization within Phaseolus has been limited by abnormal hybrid development and the rapid loss of hybridity in subsequent generations. The most advanced embryo development occurs in P. vulgaris x P. coccineus where mature seeds form. However, the reciprocal cross still results in abnormal embryos unable to reach maturity. Although hybrids can be obtained with the aid of embryo culture, abnormal embryos and plants continue to occur in subsequent generations and viable plants exhibit few hybrid or P. coccineus characteristics. This study was initiated to test the hypothesis of preferential transmission of P. vulgaris as the cause of skewed distribution of progeny phenotypes. Restriction fragment length polymorphisms (RFLPs) detected by cDNA probes were used to analyze the transmission pattern. Interspecific polymorphism was detected by 70- 86% of the 280 cDNA clones used while intraspecific polymorphism ranged between 8-37%. The segregation patterns of 63 markers complementary to 50 cDNA probes were determined in 177 F₂s of P. vulgaris x P. coccineus. Of the 28 (44%) non-Mendelian loci, 24 exhibited preferential transmission of the P. vulgaris alleles. Higher than expected frequency of maternal alleles was also recovered in reciprocal F₂s. Cytoplasmic-nuclear interactions and gametic selection may contribute to the preferential recovery of maternal alleles. The markers were mapped to nine linkage groups spanning 534 centi-Morgans. Eight non-Mendelian segregating loci including a histone H2A gene, were mapped to a single linkage group suggesting selective transmission of P. vulgaris chromosome(s).
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