Cloning, sequence and expression of the glycoprotein gene of infectious hematopoietic necrosis virus, a fish rhabdovirus Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/pc289n68w

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Double-stranded cDNA was prepared from infectious hematopoietic necrosis virus mRNA and cloned into the plasmid vector pUC8. A cDNA clone (pG8) containing the entire coding region for the glycoprotein (G-protein) of infectious hematopoietic necrosis virus was selected by hybridization to a ³²P-labeled probe. The restriction map and nucleotide sequence of the mRNA encoding the glycoprotein of infectious hematopoietic necrosis virus was determined using this full-length cDNA clone. The G-protein cDNA is 1,609 nucleotides long (excluding the polyadenylic acid) and encodes a protein of 508 amino acids. The predicted amino acid sequence was compared with that of the glycoprotein of the Indiana and New Jersey serotypes of vesicular stomatitis virus and with the glycoprotein of rabies virus, using a computer program which determined optimal alignment. An amino acid identity of approximately 20% was found between infectious hematopoietic necrosis virus and the two vesicular stomatitis virus serotypes and between infectious hematopoietic necrosis virus and rabies virus. The positions and sizes of the signal sequence and transmembrane domain and the possible glycosylation sites were determined. A cDNA fragment containing the gene encoding the glycoprotein of infectious hematopoietic necrosis virus was inserted into Autographa califomica Baculovirus vectors under the control of the polyhedrin promoter. Insect cells were cotransfected with the Baculovirus recombinants and wild-type Autographa califomica nuclear polyhedrosis virus genomic DNA. Recombinant viruses were selected by plaque assay and plaque hybridization. Gel electrophoresis and Western blot analysis revealed the presence of a 66 kilodalton protein identical in size to the glycoprotein of infectious hematopoietic necrosis virus.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 8-bit Grayscale) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Submitted by Katy Davis (kdscannerosu@gmail.com) on 2013-05-29T22:19:57Z No. of bitstreams: 1 FeyereisenKoenerJosette1988.pdf: 873784 bytes, checksum: 276d27d99119bea0d311304f25974750 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-07-16T21:17:02Z (GMT) No. of bitstreams: 1 FeyereisenKoenerJosette1988.pdf: 873784 bytes, checksum: 276d27d99119bea0d311304f25974750 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-05-30T14:46:16Z (GMT) No. of bitstreams: 1 FeyereisenKoenerJosette1988.pdf: 873784 bytes, checksum: 276d27d99119bea0d311304f25974750 (MD5)
  • description.provenance : Made available in DSpace on 2013-07-16T21:17:02Z (GMT). No. of bitstreams: 1 FeyereisenKoenerJosette1988.pdf: 873784 bytes, checksum: 276d27d99119bea0d311304f25974750 (MD5) Previous issue date: 1987-05-26

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items